LOGIN
 
Share Page:
Back

Volume 33 , Issue 1
Winter 2019

Pages 114–122


Role of Link N in Modulating Inflammatory Conditions

Mu-Chen Yang, MSc/Ding-Han Wang, PhD/Juin-Hong Cherng, PhD/Wan-Chun Li, PhD/Po-Yen Lin, DDS, PhD/Wun-Eng Hsu, DDS/Ming-Lun Hsu, DDS, Dr Med Dent


PMID: 30703176
DOI: 10.11607/ofph.1952

Aims: To elucidate the role of Link N in regulating inflammatory molecules from human mesenchymal stem cells (hMSCs) under interleukin (IL)-1β stimulation in vitro and under Complete Freund’s Adjuvant (CFA)–induced arthritis of the temporomandibular joint (TMJ) in vivo. Methods: In vitro analysis of inflammatory cytokines and epithelial-mesenchymal transition (EMT) genes in hMSCs treated with Link N, IL-1β, and co-stimulation of IL-1β and Link N was undertaken using Luminex multiplex assays and real-time polymerase chain reaction, respectively. To determine the impact of Link N in ameliorating TMJ tissue homeostasis in arthritic conditions, histologic changes in CFA-induced arthritic TMJ tissues followed by application of Link N were examined. All data were analyzed using one-way analysis of variance with Bonferroni post hoc test. Results: Increased levels of IL-6; interferon gamma-inducible protein-10; and regulated upon activation, normal T cell expressed, and secreted (RANTES) were detected in response to IL-1β treatment, but these levels were significantly decreased in the co-stimulation group. In contrast, secreted IL-4, IL-10, and transforming growth factor β1–β3 proteins, as well as intracellular erb-b2 receptor tyrosine kinase 3 and Nodal homolog genes, were increased significantly in the co-stimulation group compared to the IL-1β group. Histologic analysis showed significant recovery for rat condyle thickness in the Link N–treated group when compared to the CFA-induced arthritis group. Conclusion: These findings indicate that Link N could modulate inflammation and EMT in vitro and repair arthritis-mediated TMJ disruption in vivo. Link N could be a potential therapeutic agent for TMJ disorder patients.


Full Text PDF File | Order Article

 

 
Get Adobe Reader
Adobe Acrobat Reader is required to view PDF files. This is a free program available from the Adobe web site.
Follow the download directions on the Adobe web site to get your copy of Adobe Acrobat Reader.

 

© 2020 Quintessence Publishing Co, Inc

JOFPH Home
Current Issue
Ahead of Print
Archive
Author Guidelines
About
Submission Form
Submit
Reprints
Permission
Advertising
Quintessence Home
Terms of Use
Privacy Policy
About Us
Contact Us
Help