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The Chinese Journal of Dental Research

Year 2002
Volume 5 , Issue 2

Pages: 5 - 11

Detection and Analysis of Helicobacter Pylori in Dental Plaque and Stomach of Gastritis Patients by PCR and PCR-SSCP

Wenjie Hu, DDS, PhD/Caifang Cao, DDS, MS/Huanxin Meng, DDS, PhD/Jichang Zhang, MD, PhD/Dalong Ma, MD, PhD/Lian Zhang, MS

Objective: To investigate the presence of H. pylori in the oral cavity of gastritis patients and analyze the genotype similarity between H. pylori in the oral cavity and in the stomach. Methods: Primers based on the urease C and cag A gene sequence of H. pylori were used in a PCF assay to amplify 294-bp and 400-bp DNA fragments and the identity of amplified products was confirmed by restriction endonuclease digestion. Thirty-two patients with chronic gastritis were recruited. Twelve supra- and subgingival plaque samples were collected from 6 teeth in each subject for PCR assay. The products of urease C amplified from 12 samples of the dental plaque and stomach from 4 gastritis patients in the same family were analyzed by PCF-SSCP to reveal the genotype similarity of H. pylori. Results: Twenty-seven patients (84.4%) had at least one H. pylori-positive plaque sample; 113 (29.4%) of the 384 tested samples were urease-C-gene positive, and 9 (2.3%) were positive for both of the H. pylori genes. The H. pylori positive rate was significantly higher in subgingival plaque (7/192, 37%) than in supragingival plaque (42/192, 21.9%) (P < 0.01). The data of PCR-SSCP revealed that 3 out of 4 gastritis patients had the same straings of H. pylori as found orally, and there was at least one identical genotype of H. pylori carried by these patients. Conclusion: H. pylori are present in the oral cavity of gastritis patients. This result supports the thesis that oral H. pylori infection could be an important source of gastric infection. The cluster phenomenon and oral-oral transmission of H. pylori infection also exist.



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